Unknown

Dataset Information

0

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases.


ABSTRACT: Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450(BM3)) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted to a wide range of substituted monosaccharides and polysaccharides. Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering, suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry, biology, and medicine.

SUBMITTER: Lewis JC 

PROVIDER: S-EPMC2757845 | biostudies-other | 2009 Sep

REPOSITORIES: biostudies-other

altmetric image

Publications

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases.

Lewis Jared C JC   Bastian Sabine S   Bennett Clay S CS   Fu Yu Y   Mitsuda Yuuichi Y   Chen Mike M MM   Greenberg William A WA   Wong Chi-Huey CH   Arnold Frances H FH  

Proceedings of the National Academy of Sciences of the United States of America 20090915 39


Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450(BM3)) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted  ...[more]

Similar Datasets

| S-EPMC4004622 | biostudies-literature
| S-EPMC8163323 | biostudies-literature
| S-EPMC6563808 | biostudies-literature
| S-EPMC7649522 | biostudies-literature
| S-EPMC4278937 | biostudies-literature
| S-EPMC6436545 | biostudies-literature
| S-EPMC1144837 | biostudies-other
| S-EPMC6666315 | biostudies-literature
| S-EPMC4380956 | biostudies-literature
| S-EPMC7853949 | biostudies-literature