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A switchable light-input, light-output system modelled and constructed in yeast.

ABSTRACT: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

SUBMITTER: Sorokina O

PROVIDER: S-EPMC2758823 | biostudies-other | 2009 Sep

REPOSITORIES: biostudies-other

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A switchable light-input, light-output system modelled and constructed in yeast.

Sorokina Oxana O Kapus Anita A Terecskei Kata K Dixon Laura E LE Kozma-Bognar Laszlo L Nagy Ferenc F Millar Andrew J AJ

Journal of biological engineering 20090917

<h4>Background</h4>Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.<h4>Results</h4>The reversib ...[more]

PMID: 19761615

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Dataset Information

A switchable light-input, light-output system modelled and constructed in yeast.

Publications

A switchable light-input, light-output system modelled and constructed in yeast.

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