Unknown

Dataset Information

0

Egg coat proteins activate calcium entry into mouse sperm via CATSPER channels.


ABSTRACT: During mammalian fertilization, the contact between sperm and egg triggers increases in intracellular Ca(2+) concentration ([Ca(2+)](i)) in sperm. Voltage-gated Ca(2+) channels (Ca(V)s) are believed to mediate the initial phase of [Ca(2+)](i) increases in sperm induced by egg coat (zona pellucida [ZP]) glycoproteins, while store depletion-activated Ca(2+) entry is thought to mediate the sustained phase. Using patch-clamp recording and Ca(2+) imaging, we show herein that Ca(V) channel currents, while found in spermatogenic cells, are not detectable in epididymal sperm and are not essential for the ZP-induced [Ca(2+)](i) changes. Instead, CATSPER channels localized in the distal portion of sperm (the principal piece) are required for the ZP-induced [Ca(2+)](i) increases. Furthermore, the ZP-induced [Ca(2+)](i) increase starts from the sperm tail and propagates toward the head.

SUBMITTER: Xia J 

PROVIDER: S-EPMC2804799 | biostudies-other | 2009 Jun

REPOSITORIES: biostudies-other

altmetric image

Publications

Egg coat proteins activate calcium entry into mouse sperm via CATSPER channels.

Xia Jingsheng J   Ren Dejian D  

Biology of reproduction 20090211 6


During mammalian fertilization, the contact between sperm and egg triggers increases in intracellular Ca(2+) concentration ([Ca(2+)](i)) in sperm. Voltage-gated Ca(2+) channels (Ca(V)s) are believed to mediate the initial phase of [Ca(2+)](i) increases in sperm induced by egg coat (zona pellucida [ZP]) glycoproteins, while store depletion-activated Ca(2+) entry is thought to mediate the sustained phase. Using patch-clamp recording and Ca(2+) imaging, we show herein that Ca(V) channel currents, w  ...[more]

Similar Datasets

| S-EPMC5480393 | biostudies-literature
| S-EPMC3567151 | biostudies-literature
| S-EPMC7554638 | biostudies-literature
| S-EPMC8519427 | biostudies-literature
| S-EPMC2644657 | biostudies-literature
| S-EPMC6208766 | biostudies-literature
| S-EPMC3948348 | biostudies-literature
| S-EPMC2877556 | biostudies-literature
| S-EPMC5842911 | biostudies-literature
| S-EPMC7795802 | biostudies-literature