Ontology highlight
ABSTRACT:
SUBMITTER: Kawamoto S
PROVIDER: S-EPMC281377 | biostudies-other | 1993 Apr
REPOSITORIES: biostudies-other
Infection and immunity 19930401 4
Both Pseudomonas aeruginosa elastase and Bacillus thermoproteolyticus thermolysin are zinc metalloproteases. On the basis of the high homology of the P. aeruginosa elastase with the Bacillus thermolysin, we hypothesized that Glu-141 and His-223 are the key residues for catalytic activity of the Pseudomonas elastase. To test this possibility, we replaced Glu-141 with Asp, Gln, and Gly and His-223 with Gly, Glu, and Leu by site-directed mutagenesis. These substitutions dramatically diminished the ...[more]