Unknown

Dataset Information

0

Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.


ABSTRACT: The thyroid-stimulating hormone receptor (TSHR), activated by either TSH or the newly discovered glycoprotein hormone thyrostimulin, plays a central role in the control of body metabolism. Interestingly, in addition to its thyroid expression, we discovered that the mRNA level of TSHR is periodically regulated in rat ovary by gonadotropins. Ovarian microdissection followed by real-time PCR analysis indicated that granulosa cells show the highest level of TSHR expression. Cultures of follicles and primary granulosa cells demonstrated that the level of TSHR is up-regulated and decreased by the gonadotropin-driven cAMP cascade and estradiol production, respectively. Furthermore, in contrast to the negligible expression of TSH in the ovary, we also found by real-time PCR and immunohistochemical analysis that thyrostimulin is expressed mainly in oocytes. Evolving before the appearance of gonadotropins, thyrostimulin is considered the most ancestral glycoprotein hormone. Therefore, the presence of thyrostimulin in the ovary suggests that it may have a primitive function in reproduction when it activates ovarian TSHR. Next, we generated recombinant thyrostimulin protein and characterized its non-covalent heterodimeric nature. Using purified recombinant thyrostimulin, we show that the human ovarian cell line NIH:OVCAR-3 also expresses endogenous and functional TSHR. Using cultured rat granulosa cells isolated from different ovarian stages, we found that treatments with thyrostimulin significantly increase cAMP production and the c-fos gene response in the presence of gonadotropins. Thus, this study demonstrates that oocyte-derived thyrostimulin and granulosa cell-expressed TSHR compose a novel paracrine system in the ovary, where the activity is tightly controlled by gonadotropins.

SUBMITTER: Sun SC 

PROVIDER: S-EPMC2823517 | biostudies-other | 2010 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.

Sun Su-Chin SC   Hsu Pei-Jen PJ   Wu Fang-Ju FJ   Li Sheng-Hsiang SH   Lu Chung-Hao CH   Luo Ching-Wei CW  

The Journal of biological chemistry 20091202 6


The thyroid-stimulating hormone receptor (TSHR), activated by either TSH or the newly discovered glycoprotein hormone thyrostimulin, plays a central role in the control of body metabolism. Interestingly, in addition to its thyroid expression, we discovered that the mRNA level of TSHR is periodically regulated in rat ovary by gonadotropins. Ovarian microdissection followed by real-time PCR analysis indicated that granulosa cells show the highest level of TSHR expression. Cultures of follicles and  ...[more]

Similar Datasets

| S-EPMC3057818 | biostudies-literature
| S-EPMC3391290 | biostudies-literature
| S-EPMC3143212 | biostudies-other
| S-EPMC2713536 | biostudies-literature
| S-EPMC4249871 | biostudies-literature
| S-EPMC5712235 | biostudies-literature
| S-EPMC3830942 | biostudies-literature
| S-EPMC5376554 | biostudies-literature
| S-EPMC3775497 | biostudies-literature
| S-EPMC10577406 | biostudies-literature