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Gaussia luciferase variant for high-throughput functional screening applications.


ABSTRACT: Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.

SUBMITTER: Maguire CA 

PROVIDER: S-EPMC2846205 | biostudies-other | 2009 Aug

REPOSITORIES: biostudies-other

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Gaussia luciferase variant for high-throughput functional screening applications.

Maguire Casey A CA   Deliolanis Nikolaos C NC   Pike Lisa L   Niers Johanna M JM   Tjon-Kon-Fat Lee-Ann LA   Sena-Esteves Miguel M   Tannous Bakhos A BA  

Analytical chemistry 20090801 16


Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a sho  ...[more]

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