Dataset Information

OCT-4 expression in follicular and luteal phase endometrium: a pilot study.

ABSTRACT: BACKGROUND: The stem cell marker Octamer-4 (OCT-4) is expressed in human endometrium. Menstrual cycle-dependency of OCT-4 expression has not been investigated to date. METHODS: In a prospective, single center cohort study of 98 women undergoing hysteroscopy during the follicular (n = 49) and the luteal (n = 40) phases of the menstrual cycle, we obtained endometrial samples. Specimens were investigated for OCT-4 expression on the mRNA and protein levels using reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Expression of OCT-4 was correlated to menstrual cycle phase. RESULTS: Of 89 women sampled, 49 were in the follicular phase and 40 were in the luteal phase. OCT-4 mRNA was detected in all samples. Increased OCT-4 mRNA levels in the follicular and luteal phases was found in 35/49 (71%) and 27/40 (68%) of women, respectively (p = 0.9). Increased expression of OCT-4 protein was identified in 56/89 (63%) samples. Increased expression of OCT-4 protein in the follicular and luteal phases was found in 33/49 (67%) and 23/40 (58%) of women, respectively (p = 0.5). CONCLUSIONS: On the mRNA and protein levels, OCT-4 is not differentially expressed during the menstrual cycle. Endometrial OCT-4 is not involved in or modulated by hormone-induced cyclical changes of the endometrium.

SUBMITTER: Bentz EK

PROVIDER: S-EPMC2867815 | biostudies-other | 2010

REPOSITORIES: biostudies-other

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OCT-4 expression in follicular and luteal phase endometrium: a pilot study.

Bentz Eva-Katrin EK Kenning Marina M Schneeberger Christian C Kolbus Andrea A Huber Johannes C JC Hefler Lukas A LA Tempfer Clemens B CB

Reproductive biology and endocrinology : RB&E 20100422

<h4>Background</h4>The stem cell marker Octamer-4 (OCT-4) is expressed in human endometrium. Menstrual cycle-dependency of OCT-4 expression has not been investigated to date.<h4>Methods</h4>In a prospective, single center cohort study of 98 women undergoing hysteroscopy during the follicular (n = 49) and the luteal (n = 40) phases of the menstrual cycle, we obtained endometrial samples. Specimens were investigated for OCT-4 expression on the mRNA and protein levels using reverse transcriptase po ...[more]

PMID: 20412569

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Dataset Information

OCT-4 expression in follicular and luteal phase endometrium: a pilot study.

Publications

OCT-4 expression in follicular and luteal phase endometrium: a pilot study.

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