Ontology highlight
ABSTRACT:
SUBMITTER: Miceli C
PROVIDER: S-EPMC287055 | biostudies-other | 1989 May
REPOSITORIES: biostudies-other
Miceli C C La Terza A A Melli M M
Proceedings of the National Academy of Sciences of the United States of America 19890501 9
cDNA clones comprising the entire coding region for the mating pheromone Er-1 of Euplotes raikovi have been isolated by oligonucleotide screening of two cDNA libraries in the vectors lambda gt10 and pUC12. The cDNA sequence contains an open reading frame of 75 amino acids that constitute pre-pro-Er-1. The amino acid sequence of secreted Er-1 starts at aspartic acid-36 of pre-pro-Er-1 and completely matches that known by direct Er-1 protein sequencing. The coding region of Er-1 cDNA ends with cod ...[more]