Dataset Information

Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.

ABSTRACT: Plakoglobin is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. Using a partial cDNA clone for bovine plakoglobin, we isolated cDNAs encoding human plakoglobin, determined its nucleotide sequence, and deduced the complete amino acid sequence. The polypeptide encoded by the cDNA was synthesized by in vitro transcription and translation and identified by its comigration with authentic plakoglobin in two-dimensional gel electrophoresis. The identity was further confirmed by comparison of the deduced sequence with the directly determined amino acid sequence of two fragments from bovine plakoglobin. Analysis of the plakoglobin sequence showed the protein (744 amino acids; 81,750 Da) to be unrelated to any other known proteins, highly conserved between human and bovine tissues, and characterized by numerous changes between hydrophilic and hydrophobic sections. Only one kind of plakoglobin mRNA (3.4 kilobases) was found in most tissues, but an additional mRNA (3.7 kilobases) was detected in certain human tumor cell lines. This longer mRNA may be represented by a second type of plakoglobin cDNA, which contains an insertion of 297 nucleotides in the 3' non-coding region.

SUBMITTER: Franke WW

PROVIDER: S-EPMC287381 | biostudies-other | 1989 Jun

REPOSITORIES: biostudies-other

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Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.

Franke W W WW Goldschmidt M D MD Zimbelmann R R Mueller H M HM Schiller D L DL Cowin P P

Proceedings of the National Academy of Sciences of the United States of America 19890601 11

Plakoglobin is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. Using a partial cDNA clone for bovine plakoglobin, we isolated cDNAs encoding human plakoglobin, determined its nucleotide sequence, and deduced the complete amino acid sequence. The polypeptide encoded by the cDNA was synthesized by in vitro tran ...[more]

PMID: 2726765

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Project description:BACKGROUND: Structural flexibility is an important characteristic of proteins because it is often associated with their function. The movement of a polypeptide segment in a protein can be broken down into two types of motions: internal and external ones. The former is deformation of the segment itself, but the latter involves only rotational and translational motions as a rigid body. Normal Model Analysis (NMA) can derive these two motions, but its application remains limited because it necessitates the gathering of complete structural information. RESULTS: In this work, we present a novel method for predicting two kinds of protein motions in ordered structures. The prediction uses only information from the amino acid sequence. We prepared a dataset of the internal and external motions of segments in many proteins by application of NMA. Subsequently, we analyzed the relation between thermal motion assessed from X-ray crystallographic B-factor and internal/external motions calculated by NMA. Results show that attributes of amino acids related to the internal motion have different features from those related to the B-factors, although those related to the external motion are correlated strongly with the B-factors. Next, we developed a method to predict internal and external motions from amino acid sequences based on the Random Forest algorithm. The proposed method uses information associated with adjacent amino acid residues and secondary structures predicted from the amino acid sequence. The proposed method exhibited moderate correlation between predicted internal and external motions with those calculated by NMA. It has the highest prediction accuracy compared to a naïve model and three published predictors. CONCLUSIONS: Finally, we applied the proposed method predicting the internal motion to a set of 20 proteins that undergo large conformational change upon protein-protein interaction. Results show significant overlaps between the predicted high internal motion regions and the observed conformational change regions.

Dataset Information

Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.

Publications

Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.

Similar Datasets

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