A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods.
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ABSTRACT:
SUBMITTER: Shirran SL
PROVIDER: S-EPMC2880794 | biostudies-other | 2010 May
REPOSITORIES: biostudies-other
Publications
A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods.
Shirran Sally L SL Botting Catherine H CH
Journal of proteomics 20100315 7
The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ratios more extreme than 1:1 was investigated. A comparison of nLC-ESI MSMS and nLC-MALDI MSMS analysis routes was performed. A fixed concentration of a standard six protein mix was spiked with two proteins at a range of concentrations. The two data analysis programmes, Mascot and ProteinPilot Paragon, were also compared. Whilst the lower ratios could be measured accurately, greater discrepancies ...[more]
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