Multiphoton microscopy with near infrared contrast agents.

ABSTRACT: While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

SUBMITTER: Yazdanfar S 

PROVIDER: S-EPMC2881927 | biostudies-other | 2010 May-Jun

REPOSITORIES: biostudies-other