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A series of fluorene-based two-photon absorbing molecules: synthesis, linear and nonlinear characterization, and bioimaging.

ABSTRACT: The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imaging of COS-7 and HCT 116 cells incubated with probe I showed endosomal selectivity, demonstrating the potential of this class of fluorescent probes in multiphoton fluorescence microscopy.

SUBMITTER: Andrade CD

PROVIDER: S-EPMC2890220 | biostudies-other | 2010 Jun

REPOSITORIES: biostudies-other

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A series of fluorene-based two-photon absorbing molecules: synthesis, linear and nonlinear characterization, and bioimaging.

Andrade Carolina D CD Yanez Ciceron O CO Rodriguez Luis L Belfield Kevin D KD

The Journal of organic chemistry 20100601 12

The synthesis, structural, and photophysical characterization of a series of new fluorescent donor-acceptor and acceptor-acceptor molecules, based on the fluorenyl ring system, with two-photon absorbing properties is described. These new compounds exhibited large Stokes shifts, high fluorescence quantum yields, and significantly, high two-photon absorption cross sections, making them well suited for two-photon fluorescence microscopy imaging. Confocal and two-photon fluorescence microscopy imagi ...[more]

PMID: 20481596

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