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Rationally improving LOV domain-based photoswitches.

ABSTRACT: Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.

SUBMITTER: Strickland D

PROVIDER: S-EPMC2914111 | biostudies-other | 2010 Aug

REPOSITORIES: biostudies-other

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Rationally improving LOV domain-based photoswitches.

Strickland Devin D Yao Xiaolan X Gawlak Grzegorz G Rosen Michael K MK Gardner Kevin H KH Sosnick Tobin R TR

Nature methods 20100620 8

Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches. ...[more]

PMID: 20562867

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