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Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.

ABSTRACT: Building bridges: The use of diselenide and selectively ((15)N/(13)C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy.

SUBMITTER: Walewska A 

PROVIDER: S-EPMC2919336 | biostudies-other | 2009

REPOSITORIES: biostudies-other

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Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.

Walewska Aleksandra A   Zhang Min-Min MM   Skalicky Jack J JJ   Yoshikami Doju D   Olivera Baldomero M BM   Bulaj Grzegorz G  

Angewandte Chemie (International ed. in English) 20090101 12

Building bridges: The use of diselenide and selectively ((15)N/(13)C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy. ...[more]