Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.
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ABSTRACT:
SUBMITTER: Walewska A
PROVIDER: S-EPMC2919336 | biostudies-other | 2009
REPOSITORIES: biostudies-other
Publications
Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.
Walewska Aleksandra A Zhang Min-Min MM Skalicky Jack J JJ Yoshikami Doju D Olivera Baldomero M BM Bulaj Grzegorz G
Angewandte Chemie (International ed. in English) 20090101 12
Building bridges: The use of diselenide and selectively ((15)N/(13)C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy. ...[more]
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