The transcriptional landscape of the yeast genome defined by RNA sequencing.
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ABSTRACT:
SUBMITTER: Nagalakshmi U
PROVIDER: S-EPMC2951732 | biostudies-other | 2008 Jun
REPOSITORIES: biostudies-other
Publications
The transcriptional landscape of the yeast genome defined by RNA sequencing.
Nagalakshmi Ugrappa U Wang Zhong Z Waern Karl K Shou Chong C Raha Debasish D Gerstein Mark M Snyder Michael M
Science (New York, N.Y.) 20080501 5881
The identification of untranslated regions, introns, and coding regions within an organism remains challenging. We developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and mapped to the genome. We applied RNA-Seq to generate a high-resolution transcriptome map of the yeast genome and demonstrated that most (74.5%) of the nonrepetitive sequence of the yeast genome is transc ...[more]
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