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Molecular cloning and sequence analysis of chum salmon gonadotropin cDNAs.

ABSTRACT: cDNAs encoding alpha and beta subunits of salmon gonadotropins, sGTHI and sGTHII, have been isolated from the cDNA library prepared from salmon pituitary mRNA. sGTHI alpha, sGTHI beta, and sGTHII beta cDNAs encode polypeptides of 114, 137, and 142 amino acids, including signal peptides of 22, 24, and 23 amino acids, respectively. The deduced amino acid sequence for sGTHI alpha revealed rather high homology (66-69%) to mammalian alpha chains, whereas sGTHI beta and sGTHII beta show lower homology (30%) to each other and to mammalian beta subunits. The existence of two distinct beta-subunit cDNAs in the teleost suggests that divergence of the GTH gene took place earlier than divergence of teleosts from the main line of evolution leading to tetrapods.

SUBMITTER: Sekine S

PROVIDER: S-EPMC298344 | biostudies-other | 1989 Nov

REPOSITORIES: biostudies-other

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Molecular cloning and sequence analysis of chum salmon gonadotropin cDNAs.

Sekine S S Saito A A Itoh H H Kawauchi H H Itoh S S

Proceedings of the National Academy of Sciences of the United States of America 19891101 22

cDNAs encoding alpha and beta subunits of salmon gonadotropins, sGTHI and sGTHII, have been isolated from the cDNA library prepared from salmon pituitary mRNA. sGTHI alpha, sGTHI beta, and sGTHII beta cDNAs encode polypeptides of 114, 137, and 142 amino acids, including signal peptides of 22, 24, and 23 amino acids, respectively. The deduced amino acid sequence for sGTHI alpha revealed rather high homology (66-69%) to mammalian alpha chains, whereas sGTHI beta and sGTHII beta show lower homology ...[more]

PMID: 2813416

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Project description:Snake venom metalloproteinases (SVMPs) are a superfamily of zinc-dependent proteases and participate in a number of important biological, physiological and pathophysiological processes. In this work, we simultaneously amplified nine cDNAs encoding different classes of metalloproteinases from glands of four different snake species (Agkistrodon contortrix laticinctus, Crotalus atrox, Crotalus viridis viridis and Agkistrodon piscivorus leucostoma) by RT-PCR with a pair of primers. Among the encoded metalloproteinases, two enzymes (AclVMP-I and AplVMP-I), three enzymes (CaVMP-II, CvvVMP-II and AplVMP-II) and four enzymes (AclVMP-III, CaVMP-III, CvvVMP-III and AplVMP-III) with the characteristic motif (HEXXHXXGXXH) of metalloproteinase belong to type P-I, P-II and P-III enzymes, respectively. Disintegrin domains of CaVMP-II and CvvVMP-II from two Crotatus snakes contain RGD-motif whereas AplVMP-II from Agkistrodon snake has KGD-motif. Instead of R/KGD-motif within disintegrin domain of SVMP-II enzyme, CaVMP-III, CvvVMP-III and AplVMP-III enzymes contain SECD-motif, while AclVMP-III has DDCD-motif in their corresponding position of disintegrin-like domains. There are 12 Cys amino acids in cysterin-rich domains of each P-III enzyme. Moreover, a disintegrin precursor (AplDis) with RGD-motif also simultaneously amplified from the glands of A.p. leucostoma while amplifying AplVMP-II and AplVMP-III, which indicated that different types of SVMPs and related genes are present in a single species of snake and share a consensus sequence at the 3' and 5' untranslated regions. RT-PCR result also showed that P-III is highly expressed in Crotalus snakes than in Agkistrodon snakes. Aligning the deduced amino acid sequence of these enzymes with other SVMPs from GenBank database indicated that this is the first report on the isolation of cDNAs encoding P-II and P-III enzymes from C.v. viridis and A.p. leucostoma snakes. The availability of these SVMP sequences directly facilitated further studies of structure characterization and diversified function analysis.

Dataset Information

Molecular cloning and sequence analysis of chum salmon gonadotropin cDNAs.

Publications

Molecular cloning and sequence analysis of chum salmon gonadotropin cDNAs.

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