Ontology highlight
ABSTRACT:
SUBMITTER: Vo NK
PROVIDER: S-EPMC2984217 | biostudies-other | 2010 Nov
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20101025 45
Predictions of microRNA-mRNA interactions typically rely on bioinformatic algorithms, but these algorithms only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. We developed an affinity purification approach to empirically identify microRNAs associated with the 3'UTR of the mRNA encoding Hand2, a transcription factor essential for cardiac development. In addition to miR-1, a known regulator of Hand2 expression, we determined that ...[more]