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Isolation and characterization of s-myc, a member of the rat myc gene family.

ABSTRACT: A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing that the s-myc gene contains an open reading frame consisting of 1287 base pairs without introns. In vitro transcription-translation analysis of s-myc indicated that this gene produces a protein of approximately 50 kDa. The amino acid sequence predicted from the DNA sequence showed that the s-Myc protein is closely related to the murine N-Myc protein but lacks an acidic amino acid-rich sequence commonly present in the Myc-family proteins. Studies on transfection of s-myc into rat RT4-AC tumor cells revealed that the gene produces a polyadenylylated transcript of approximately 4.7 kilobases and that its high-level expression suppresses the tumor-igenicity of RT4-AC tumor cells in nude mice.

SUBMITTER: Sugiyama A

PROVIDER: S-EPMC298450 | biostudies-other | 1989 Dec

REPOSITORIES: biostudies-other

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Isolation and characterization of s-myc, a member of the rat myc gene family.

Sugiyama A A Kume A A Nemoto K K Lee S Y SY Asami Y Y Nemoto F F Nishimura S S Kuchino Y Y

Proceedings of the National Academy of Sciences of the United States of America 19891201 23

A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing that the s-myc gene contains an open reading frame consisting of 1287 base pairs without introns. In vitro transcription-translation analysis of s-myc indicated that this gene produces a protein of approximately 50 kDa. The amino acid sequence predicted from the DNA sequence showed that the s-Myc prot ...[more]

PMID: 2594755

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