Ontology highlight
ABSTRACT:
SUBMITTER: Horvath A
PROVIDER: S-EPMC2995087 | biostudies-other | 2010 Nov
REPOSITORIES: biostudies-other
Horváth András A Vértessy Beáta G BG
Nucleic acids research 20100922 21
Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The archaeal DNA polymerase from Pyrococcus furiosus binds strongly to the deaminated base uracil and stalls on uracil-containing templates. Here, we present a straightforward method for quantitative asse ...[more]