Ontology highlight
ABSTRACT:
SUBMITTER: Gangadharan S
PROVIDER: S-EPMC3009821 | biostudies-other | 2010 Dec
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20101203 51
Transposons are mobile genetic elements that are an important source of genetic variation and are useful tools for genome engineering, mutagenesis screens, and vectors for transgenesis including gene therapy. We have used second-generation sequencing to analyze ≈2 × 10(5) unique de novo transposon insertion sites of the transposon Hermes in the Saccharomyces cerevisiae genome from both in vitro transposition reactions by using purified yeast genomic DNA, to better characterize intrinsic sequence ...[more]