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Dissociation of bimolecular ?IIb?3-fibrinogen complex under a constant tensile force.

ABSTRACT: The regulated ability of integrin ?IIb?3 to bind fibrinogen plays a crucial role in platelet aggregation, adhesion, and hemostasis. Employing an optical-trap-based electronic force clamp, we studied the thermodynamics and kinetics of ?IIb?3-fibrinogen bond formation and dissociation under constant unbinding forces, mimicking the forces of physiologic blood shear on a thrombus. The distribution of bond lifetimes was bimodal, indicating that the ?IIb?3-fibrinogen complex exists in two bound states with different mechanical stability. The ?IIb?3 antagonist, abciximab, inhibited binding without affecting the unbinding kinetics, whereas Mn²(+) biased the ?IIb?3-fibrinogen complex to the strong bound state with reduced off-rate. The average bond lifetimes decreased exponentially with increasing pulling force from ?5 pN to 50 pN, suggesting that in this force range the ?IIb?3-fibrinogen interactions are classical slip bonds. We found no evidence for catch bonds, which is consistent with the known lack of shear-enhanced platelet adhesion on fibrinogen-coated surfaces. Taken together, these data provide important quantitative and qualitative characteristics of ?IIb?3-fibrinogen binding and unbinding that underlie the dynamics of platelet adhesion and aggregation in blood flow.

SUBMITTER: Litvinov RI 

PROVIDER: S-EPMC3010843 | biostudies-other | 2011 Jan

REPOSITORIES: biostudies-other

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Dissociation of bimolecular αIIbβ3-fibrinogen complex under a constant tensile force.

Litvinov Rustem I RI   Barsegov Valeri V   Schissler Andrew J AJ   Fisher Andrew R AR   Bennett Joel S JS   Weisel John W JW   Shuman Henry H  

Biophysical journal 20110101 1

The regulated ability of integrin αIIbβ3 to bind fibrinogen plays a crucial role in platelet aggregation, adhesion, and hemostasis. Employing an optical-trap-based electronic force clamp, we studied the thermodynamics and kinetics of αIIbβ3-fibrinogen bond formation and dissociation under constant unbinding forces, mimicking the forces of physiologic blood shear on a thrombus. The distribution of bond lifetimes was bimodal, indicating that the αIIbβ3-fibrinogen complex exists in two bound states  ...[more]