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Rapid tagging and integration of genes in Giardia intestinalis.

ABSTRACT: We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.

SUBMITTER: Gourguechon S 

PROVIDER: S-EPMC3019802 | biostudies-other | 2011 Jan

REPOSITORIES: biostudies-other

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Rapid tagging and integration of genes in Giardia intestinalis.

Gourguechon Stéphane S   Cande W Zacheus WZ  

Eukaryotic cell 20101129 1

We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized. ...[more]