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A landmark-free morphometric staging system for the mouse limb bud.


ABSTRACT: We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about ±2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns.

SUBMITTER: Boehm B 

PROVIDER: S-EPMC3042875 | biostudies-other | 2011 Mar

REPOSITORIES: biostudies-other

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A landmark-free morphometric staging system for the mouse limb bud.

Boehm Bernd B   Rautschka Michael M   Quintana Laura L   Raspopovic Jelena J   Jan Ziga Z   Sharpe James J  

Development (Cambridge, England) 20110209 6


We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about ±2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and ther  ...[more]

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