Trans-activation and DNA-binding properties of the transcription factor, Sox-18.
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ABSTRACT:
SUBMITTER: Hosking BM
PROVIDER: S-EPMC307084 | biostudies-other | 1995 Jul
REPOSITORIES: biostudies-other
Publications
Trans-activation and DNA-binding properties of the transcription factor, Sox-18.
Hosking B M BM Muscat G E GE Koopman P A PA Dowhan D H DH Dunn T L TL
Nucleic acids research 19950701 14
Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene). We have bacterially expressed this 378 amino acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160-225). Furthermore, Sox-18 was capable of trans-activating gene expression through the AACAAA motif. Our results suggest that Sox-18 functions as a classical trans-activator of ge ...[more]
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