Quantification of biological interactions with particle image cross-correlation spectroscopy (PICCS).
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ABSTRACT:
SUBMITTER: Semrau S
PROVIDER: S-EPMC3072609 | biostudies-other | 2011 Apr
REPOSITORIES: biostudies-other
Publications
Quantification of biological interactions with particle image cross-correlation spectroscopy (PICCS).
Semrau Stefan S Holtzer Laurent L González-Gaitán Marcos M Schmidt Thomas T
Biophysical journal 20110401 7
A multitude of biological processes that involve multiple interaction partners are observed by two-color microscopy. Here we describe an analysis method for the robust quantification of correlation between signals in different color channels: particle image cross-correlation spectroscopy (PICCS). The method, which exploits the superior positional accuracy obtained in single-object and single-molecule microscopy, can extract the correlation fraction and length scale. We applied PICCS to correlati ...[more]
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