Ontology highlight
ABSTRACT:
SUBMITTER: Bradshaw MS
PROVIDER: S-EPMC307474 | biostudies-other | 1995 Dec
REPOSITORIES: biostudies-other
Bradshaw M S MS Bollekens J A JA Ruddle F H FH
Nucleic acids research 19951201 23
The functional analysis of genes frequently requires manipulation of large genomic regions embedded in yeast artificial chromosomes (YACs). We have designed a yeast-bacteria shuttle vector, pClasper, that can be used to clone specific regions of interest from YACs by homologous recombination. The important feature of pClasper is the presence of the mini-F factor replicon. This leads to a significant increase in the size of the plasmid inserts that can be maintained in bacteria after cloning by h ...[more]