Ontology highlight
ABSTRACT:
SUBMITTER: Yang Q
PROVIDER: S-EPMC308533 | biostudies-other | 1994 Nov
REPOSITORIES: biostudies-other
Yang Q Q Zwick M G MG Paule M R MR
Nucleic acids research 19941101 22
The primary sequence of the entire 2330 bp intergenic spacer of the A.castellanii ribosomal RNA gene was determined. Repeated sequence elements averaging 140 bp were identified and found to bind a protein required for optimum initiation at the core promoter. These repeated elements were shown to stimulate rRNA transcription by RNA polymerase I in vitro. The repeats inhibited transcription when placed in trans, and stimulated transcription when in cis, in either orientation, but only when upstrea ...[more]