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Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11.

ABSTRACT: POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oct-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis. In addition, it is abundant in the myeloma cell line P3/NS-1/1-Ag4.1. We describe the structure of Oct-11 and its chromosomal localization, and discuss the evidence that the POU class II gene family has evolved by duplication and divergence of a common ancestral gene.

SUBMITTER: Goldsborough AS

PROVIDER: S-EPMC309074 | biostudies-other | 1993 Jan

REPOSITORIES: biostudies-other

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Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11.

Goldsborough A S AS Healy L E LE Copeland N G NG Gilbert D J DJ Jenkins N A NA Willison K R KR Ashworth A A

Nucleic acids research 19930101 1

POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oct-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult ...[more]

PMID: 8441607

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