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Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme.

ABSTRACT: The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.

SUBMITTER: Carter JR

PROVIDER: S-EPMC309768 | biostudies-other | 1993 Jul

REPOSITORIES: biostudies-other

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Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme.

Carter J R JR Franden M A MA Aebersold R R Kim D R DR McHenry C S CS

Nucleic acids research 19930701 14

The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit fro ...[more]

PMID: 8341603

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