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Transcriptional repressor ZF5 identifies a new conserved domain in zinc finger proteins.

ABSTRACT: We have cloned a cDNA encoding a new murine C2H2 zinc finger protein, ZF5. The 51.3 kD protein contains five GL1-Kruppel type zinc fingers at the C-terminus. At its N-terminus, ZF5 has a 41 amino acid region which was found to be homologous to the N-termini of several other zinc finger proteins. This region defines a new motif within zinc finger proteins which we have named the Zinc finger N-terminal (ZiN) domain. ZF5 binds to two sites in the c-myc promoter and to the -50 bp site of the herpes simplex thymidine kinase promoter. ZF5 is a transcriptional repressor and its repression domain is located N-terminal to the zinc finger domains. A single 4 kb ZF5 mRNA is expressed widely.

SUBMITTER: Numoto M

PROVIDER: S-EPMC309887 | biostudies-other | 1993 Aug

REPOSITORIES: biostudies-other

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Transcriptional repressor ZF5 identifies a new conserved domain in zinc finger proteins.

Numoto M M Niwa O O Kaplan J J Wong K K KK Merrell K K Kamiya K K Yanagihara K K Calame K K

Nucleic acids research 19930801 16

We have cloned a cDNA encoding a new murine C2H2 zinc finger protein, ZF5. The 51.3 kD protein contains five GL1-Kruppel type zinc fingers at the C-terminus. At its N-terminus, ZF5 has a 41 amino acid region which was found to be homologous to the N-termini of several other zinc finger proteins. This region defines a new motif within zinc finger proteins which we have named the Zinc finger N-terminal (ZiN) domain. ZF5 binds to two sites in the c-myc promoter and to the -50 bp site of the herpes ...[more]

PMID: 8367294

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