Ontology highlight
ABSTRACT:
SUBMITTER: Dimitrova D
PROVIDER: S-EPMC310515 | biostudies-other | 1993 Dec
REPOSITORIES: biostudies-other
Dimitrova D D Vassilev L L Anachkova B B Russev G G
Nucleic acids research 19931201 24
By using an original two-step technique (trioxsalen crosslinking/immunoprecipitation) we were able to isolate in a single-stranded form a fraction of mouse DNA enriched in putative Replication Initiation Sequences (RIS). The isolated and purified single-strand fragments were made double-stranded in vitro and were cloned in pUC12 to prepare a confined RIS library. 30 randomly selected RIS inserts were subjected to gel mobility shift assay using nuclear extracts either from dividing, or from quies ...[more]