Ontology highlight
ABSTRACT:
SUBMITTER: Crisell P
PROVIDER: S-EPMC310644 | biostudies-other | 1993 Nov
REPOSITORIES: biostudies-other
Crisell P P Thompson S S James W W
Nucleic acids research 19931101 22
Self-cleaving RNAs (ribozymes) can be engineered to cleave target RNAs of choice in a sequence-specific manner (1). Consequently, they could be used to inhibit virus replication or to analyse host gene function in vivo. However, ribozymes that are catalytic in vitro are generally disappointing when analysed in cells unless expressed at high levels relative to their target RNAs (2, 3). Here we provide evidence that this can be overcome by optimizing ribozyme structure using cellular rather than c ...[more]