Ontology highlight
ABSTRACT:
SUBMITTER: Chinai JM
PROVIDER: S-EPMC3110995 | biostudies-other | 2011 Jun
REPOSITORIES: biostudies-other
Chinai Jordan M JM Taylor Alexander B AB Ryno Lisa M LM Hargreaves Nicholas D ND Morris Christopher A CA Hart P John PJ Urbach Adam R AR
Journal of the American Chemical Society 20110407 23
The discovery of molecules that bind tightly and selectively to desired proteins continues to drive innovation at the interface of chemistry and biology. This paper describes the binding of human insulin by the synthetic receptor cucurbit[7]uril (Q7) in vitro. Isothermal titration calorimetry and fluorescence spectroscopy experiments show that Q7 binds to insulin with an equilibrium association constant of 1.5 × 10(6) M(-1) and with 50-100-fold selectivity versus proteins that are much larger bu ...[more]