Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.
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ABSTRACT:
SUBMITTER: Nazir KH
PROVIDER: S-EPMC3126401 | biostudies-other | 2011 May
REPOSITORIES: biostudies-other
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Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.
Nazir K H M Nazmul Hussain KH Ichinose Hirofumi H Wariishi Hiroyuki H
Applied and environmental microbiology 20110304 9
A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs. ...[more]
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