Ontology highlight
ABSTRACT:
SUBMITTER: Zustiak SP
PROVIDER: S-EPMC3127197 | biostudies-other | 2011 Jul
REPOSITORIES: biostudies-other
Zustiak Silviya P SP Nossal Ralph R Sackett Dan L DL
Biophysical journal 20110701 1
Diffusion of molecules in the crowded and charged interior of the cell has long been of interest for understanding cellular processes. Here, we introduce a model system of hindered diffusion that includes both crowding and binding. In particular, we obtained the diffusivity of the positively charged protein, ribonuclease A (RNase), in solutions of dextrans of various charges (binding) and concentrations (crowding), as well as combinations of both, in a buffer of physiological ionic strength. Usi ...[more]