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Methionine sulfoxide reductase A is a stereospecific methionine oxidase.

ABSTRACT: Methionine sulfoxide reductase A (MsrA) catalyzes the reduction of methionine sulfoxide to methionine and is specific for the S epimer of methionine sulfoxide. The enzyme participates in defense against oxidative stresses by reducing methionine sulfoxide residues in proteins back to methionine. Because oxidation of methionine residues is reversible, this covalent modification could also function as a mechanism for cellular regulation, provided there exists a stereospecific methionine oxidase. We show that MsrA itself is a stereospecific methionine oxidase, producing S-methionine sulfoxide as its product. MsrA catalyzes its own autooxidation as well as oxidation of free methionine and methionine residues in peptides and proteins. When functioning as a reductase, MsrA fully reverses the oxidations which it catalyzes.

SUBMITTER: Lim JC

PROVIDER: S-EPMC3127874 | biostudies-other | 2011 Jun

REPOSITORIES: biostudies-other

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Methionine sulfoxide reductase A is a stereospecific methionine oxidase.

Lim Jung Chae JC You Zheng Z Kim Geumsoo G Levine Rodney L RL

Proceedings of the National Academy of Sciences of the United States of America 20110613 26

Methionine sulfoxide reductase A (MsrA) catalyzes the reduction of methionine sulfoxide to methionine and is specific for the S epimer of methionine sulfoxide. The enzyme participates in defense against oxidative stresses by reducing methionine sulfoxide residues in proteins back to methionine. Because oxidation of methionine residues is reversible, this covalent modification could also function as a mechanism for cellular regulation, provided there exists a stereospecific methionine oxidase. We ...[more]

PMID: 21670260

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