Kruppel-like factor KLF9 regulates PPAR? transactivation at the middle stage of adipogenesis.

ABSTRACT: Krüppel-like factors (KLFs) as a family of zinc-finger transcription factors involve in the regulation of many physiological processes. In these studies, KLF9 was characterized for its role in adipogenesis. The expression of KLF9 was markedly upregulated during the middle stage of 3T3-L1 adipocyte differentiation, and inhibition of KLF9 by RNAi impaired adipogenesis. Using promoter deletion and mutation analysis, we identified two KLF9-binding sites within the 0.6-kb region of the PPAR?2 proximal promoter, indicating that KLF9 interacts with the PPAR?2 promoter. Furthermore, we found that KLF9 could synergistically activate PPAR?2 promoter by directly interacting with C/EBP?. In addition, overexpression of PPAR?2 rescued the impairment of adipocyte differentiation induced by KLF9 knockdown, which supports that PPAR?2 is a downstream target of KLF9. Collectively, our results indicate KLF9 as a key pro-adipogenic transcription factor through regulation of PPAR?2 expression with C/EBP? at the middle stage of adipogenesis.

SUBMITTER: Pei H 

PROVIDER: S-EPMC3131894 | biostudies-other | 2011 Feb

REPOSITORIES: biostudies-other