Ontology highlight
ABSTRACT:
SUBMITTER: Liu CY
PROVIDER: S-EPMC3149268 | biostudies-other | 2011 May
REPOSITORIES: biostudies-other
Liu Chen-Yu CY Qureshi Mohd Tanvir MT Lee Tae-Hee TH
Biophysical journal 20110501 9
Transfer RNA (tRNA) translocates inside the ribosome during translation. We studied the interaction strengths between the ribosome and tRNA at various stages of translocation. We utilized an optical trap to measure the mechanical force to rupture tRNA from the ribosome. We measured the rupture forces of aminoacyl tRNA or peptidyl tRNA mimic from the ribosome in a prepeptidyl transfer state, the pretranslocational state, and the posttranslocational state. In addition, we measured the interaction ...[more]