Unknown

Dataset Information

0

DNA methylation profiling in nanochannels.


ABSTRACT: We report the profiling of the 5-methyl cytosine distribution within single genomic-sized DNA molecules at a gene-relevant resolution. This method linearizes and stretches DNA molecules by confinement to channels with a dimension of about 250×200 nm(2). The methylation state is detected using fluorescently labeled methyl-CpG binding domain proteins (MBD), with high signal contrast and low background. DNA barcodes consisting of methylated and non-methylated segments are generated, with both short and long concatemers demonstrating spatially resolved MBD binding. The resolution of the technique is better than 10 kbp, and single-molecule read-lengths exceeding 140 kbp have been achieved.

SUBMITTER: Lim SF 

PROVIDER: S-EPMC3161501 | biostudies-other | 2011 Sep

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6953351 | biostudies-literature
| S-EPMC8144238 | biostudies-literature
| S-EPMC7819999 | biostudies-literature
| S-EPMC3150045 | biostudies-literature
| S-EPMC9496079 | biostudies-literature
| S-EPMC2762475 | biostudies-literature
| S-EPMC7282965 | biostudies-literature
| S-EPMC6395348 | biostudies-literature
| S-EPMC10546043 | biostudies-literature
| S-EPMC1345696 | biostudies-literature