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Media composition influences yeast one- and two-hybrid results.

ABSTRACT: Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may optimize signal differences for individual experiments, media preparation must be reported in detail to replicate studies and accurately compare results from different experiments.

SUBMITTER: Liu Y

PROVIDER: S-EPMC3177868 | biostudies-other | 2011 Aug

REPOSITORIES: biostudies-other

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Media composition influences yeast one- and two-hybrid results.

Liu Ying Y Merchant Zabeena Z Hsiao Hao-Ching HC Gonzalez Kim L KL Matthews Kathleen S KS Bondos Sarah E SE

Biological procedures online 20110815

Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may ...[more]

PMID: 21843345

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