Dataset Information

Metabolic Characterization of cold active Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium spp. from Western Himalayas.

ABSTRACT: UNLABELLED: Himalayan soils undergo dramatic temporal changes in their microclimatic properties. The soil habitats in the high altitude cold habitats of Himalayas are little explored with respect to bacterial diversity and metabolic potentials of the bacterial species. Soil habitat in Western Himalayas is dominated by the genera of Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium. Strains were found to be diverse in their metabolic potentials to utilize different carbon sources by growing them on media containing 114 different sole carbon sources. Bacillus sp. STL9 was supported by the lowest number (12.3%) of the carbon sources while growth was observed in 73.7% of the carbon sources tested for the Pseudomonas sp. SPS2. Carbohydrates appeared to be preferred carbon sources for these Himalayan isolates followed by amino acids and proteins. These microbes also produced various extra-cellular hydrolytic enzymes having biotechnological potentials, lipase being the one secreted by most strains (85.7%) followed by β-galactosidase (42.8%). Antibiotic resistance profiling for 85 different antibiotics has also been described. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12088-011-0092-7) contains supplementary material, which is available to authorized users.

SUBMITTER: Gangwar P

PROVIDER: S-EPMC3209869 | biostudies-other | 2011 Jan

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

Metabolic Characterization of cold active Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium spp. from Western Himalayas.

Gangwar Pooja P Alam Syed Imteyaz SI Singh Lokendra L

Indian journal of microbiology 20110126 1

<h4>Unlabelled</h4>Himalayan soils undergo dramatic temporal changes in their microclimatic properties. The soil habitats in the high altitude cold habitats of Himalayas are little explored with respect to bacterial diversity and metabolic potentials of the bacterial species. Soil habitat in Western Himalayas is dominated by the genera of Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium. Strains were found to be diverse in their metabolic potentials to utilize different carbon sources by ...[more]

PMID: 22282631

Similar Datasets

Project description:Chickpea is one of the most important grain legume crops in the world. India is the largest producer, consumer as well as importer of chickpea. Cold stress (temperature < 15 °C) is one of the important abiotic stresses limiting chickpea production by hampering its growth and vigor at all phenological stages. This study was aimed to characterize a diverse set of 366 chickpea genotypes for cold tolerance and identify most promising cold tolerant chickpea genotypes in the Western-Himalayas of Jammu and Kashmir, India. The 366 genotypes used during the present study including genotypes belonging to cultivated, primary and secondary gene pools of chickpea. Two important approaches were used including visual screening under field conditions and screening under controlled conditions by measuring cell membrane stability through electrolyte leakage tests. The analysis of trait data collected through both the approaches led to the identification of five most promising/candidate cold tolerant chickpea genotypes including one wild genotype "Ortan-066" from secondary gene pool species (C. echinospermum), one wild genotype "Cudi 1-022" from primary gene pool species (C. reticulatum) and three genotypes (IC 116783, ICC 15200 and AGBLG 170004) from the cultivated species (Cicer arietinum). Wild genotype "Ortan-066" was found best cold tolerance source with the mean Cold Tolerance Rating (CTR) of 2 and Electrolyte Leakage Index (ELI) of 10.82%, followed by wild genotype "Cudi 1-022" (CTR = 3, ELI = 18.89%), and three cultivated genotypes viz., IC 116783, ICC 15200 and AGBL-G-170004, with the mean CTR of 3 and an estimated mean ELI of 21.26%, 21.58% and 21.94%, respectively. The promising, candidate cold tolerant genotypes identified during the present study could be used in chickpea breeding programs aimed at improving cold tolerance of cultivated chickpea worldwide. The candidate lines can be also used for developing bi-parental mapping populations, wild × cultivated introgression lines, transcriptomics and for differential expression analysis of cold tolerant genes in chickpea.Supplementary informationThe online version contains supplementary material available at 10.1007/s12298-021-00997-1.

Project description:The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens Alternaria solani, Fusarium solani (BCCM-MUCL 5492), Pectobacterium carotovorum (ATCC 15713), Phytophthora infestans (CRA-W10022) and Rhizoctonia solani (BCCM-MUCL 51929) were performed, allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of Bacillus pumilus strains toward P. infestans, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven Bacillus spp. and four Pseudomonas spp.) in order to evaluate their in vivo antagonistic effect against P. infestans. Based on these results, four strains (Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8) were retained for further evaluation of their protection index against P. infestans in a pilot field trial. Interestingly, B. subtilis 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.

Dataset Information

Metabolic Characterization of cold active Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium spp. from Western Himalayas.

Publications

Metabolic Characterization of cold active Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium spp. from Western Himalayas.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets