A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.
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ABSTRACT:
SUBMITTER: Calleja F
PROVIDER: S-EPMC321990 | biostudies-other | 1985 Sep
REPOSITORIES: biostudies-other
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A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.
Calléja F F Tandeau de Marsac N N Coursin T T van Ormondt H H de Waard A A
Nucleic acids research 19850901 18
A new sequence-specific endonuclease from the cyanobacterium Synechocystis species PCC 6701 has been purified and characterized. This enzyme, SecI, is unique in recognizing the nucleotide sequence: 5' -CCNNGG-3' 3' -GGNNCC-5' and cleaves it at the position indicated by the symbol. Two other restriction endonucleases, SecII and SecIII, found in this organism are isoschizomers of MspI and MstII, respectively. ...[more]
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