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Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2).

ABSTRACT: The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.

SUBMITTER: Enkhbaatar B 

PROVIDER: S-EPMC3255728 | biostudies-other | 2012 Jan

REPOSITORIES: biostudies-other

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Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2).

Enkhbaatar Bolormaa B   Temuujin Uyangaa U   Lim Ju-Hyeon JH   Chi Won-Jae WJ   Chang Yong-Keun YK   Hong Soon-Kwang SK  

Applied and environmental microbiology 20111118 2

The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues. ...[more]

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