Unknown

Dataset Information

0

The sequence flanking translational initiation site in protozoa.


ABSTRACT: Nucleotide sequences flanking the translational initiation site were compiled from protozoan nuclear genes and were compared in every protozoan group. The entire 5'-untranslated sequences were very rich in A- and T-residues, but poor in G- and C-residues in most protozoan genes except for the flagellated ones. The sequence AAAAATTTTTAAAATTTAAAATGANAT emerged as a consensus sequence flanking the initiation site in the major protozoan group, although the sequences upstream from -4 (four nucleotides upstream from the ATG codon) were divergent among ciliates, sarcodinians, and sporozoans. On the other hand, the consensus sequence for flagellates was revealed to be a simple feature. Only the nucleotide position -3 was occupied with a high frequency of A-residue, in other positions it appeared randomly. These facts suggest that the strong preference for A-residue at the position -3 is a universal feature in nuclear genes for all eukaryotes.

SUBMITTER: Yamauchi K 

PROVIDER: S-EPMC328191 | biostudies-other | 1991 May

REPOSITORIES: biostudies-other

altmetric image

Publications

The sequence flanking translational initiation site in protozoa.

Yamauchi K K  

Nucleic acids research 19910501 10


Nucleotide sequences flanking the translational initiation site were compiled from protozoan nuclear genes and were compared in every protozoan group. The entire 5'-untranslated sequences were very rich in A- and T-residues, but poor in G- and C-residues in most protozoan genes except for the flagellated ones. The sequence AAAAATTTTTAAAATTTAAAATGANAT emerged as a consensus sequence flanking the initiation site in the major protozoan group, although the sequences upstream from -4 (four nucleotide  ...[more]

Similar Datasets

| S-EPMC2190805 | biostudies-other
2010-05-16 | E-GEOD-16540 | biostudies-arrayexpress
| S-EPMC525683 | biostudies-literature
| S-EPMC9530848 | biostudies-literature
| S-EPMC7490824 | biostudies-literature
2009-07-16 | GSE16540 | GEO
2018-06-18 | GSE97689 | GEO
| S-EPMC5461347 | biostudies-literature
| S-EPMC5887309 | biostudies-literature
| S-EPMC6097692 | biostudies-literature