Ontology highlight
ABSTRACT:
SUBMITTER: Weber CK
PROVIDER: S-EPMC328331 | biostudies-other | 1991 Jun
REPOSITORIES: biostudies-other
Weber C K CK Shaffer D J DJ Sidman C L CL
Nucleic acids research 19910601 12
Denaturing gradient gel electrophoresis (DGGE) is based upon the different melting behaviors of DNA molecules in a chemical denaturant gradient according to their sequences. This technique has recently become a widespread tool to detect mutations. The introduction of a GC-clamp enables the detection of most single base differences between two DNA molecules. As a test system we have applied the polymerase chain reaction (PCR) in combination with DGGE to detect a number of mutations in the mouse H ...[more]