Approaches to localizing disease genes as applied to cystic fibrosis.
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ABSTRACT:
SUBMITTER: Dean M
PROVIDER: S-EPMC330273 | biostudies-other | 1990 Jan
REPOSITORIES: biostudies-other
Publications
Approaches to localizing disease genes as applied to cystic fibrosis.
Dean M M Drumm M L ML Stewart C C Gerrard B B Perry A A Hidaka N N Cole J L JL Collins F S FS Iannuzzi M C MC
Nucleic acids research 19900101 2
Using chromosome jumping and walking and restriction fragment length polymorphism (RFLP) analysis, we have defined the region which must contain the cystic fibrosis gene. DNA segments spanning approximately 250 kb in the direction of the gene were isolated and used to identify several new polymorphisms informative in cystic fibrosis families. These RFLPs include a highly polymorphic, CA/GT repeat, and a 10 bp insertion uncovered using the polymerase chain reaction. By analyzing a family with a r ...[more]
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