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A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.

ABSTRACT: Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' followed by a strongly curved DNA sequence that exhibits weak matrix binding. DNA curvature is not a physical property required for strong matrix binding. Possible biological functions of this sequence arrangement, particularly of the strongly curved DNA, are discussed.

SUBMITTER: von Kries JP

PROVIDER: S-EPMC331089 | biostudies-other | 1990 Jul

REPOSITORIES: biostudies-other

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A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.

von Kries J P JP Phi-Van L L Diekmann S S Strätling W H WH

Nucleic acids research 19900701 13

Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' followed by a strongly curved DNA sequence that exhibits weak matrix binding. DNA curvature is not a physical property required for strong matrix binding. Possible biological functions of this sequence arrangement ...[more]

PMID: 2374712

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