Ontology highlight
ABSTRACT:
SUBMITTER: Niers JM
PROVIDER: S-EPMC3310895 | biostudies-other | 2012 Mar
REPOSITORIES: biostudies-other
Niers Johanna M JM Chen John W JW Lewandrowski Grant G Kerami Mariam M Garanger Elisabeth E Wojtkiewicz Greg G Waterman Peter P Keliher Edmund E Weissleder Ralph R Tannous Bakhos A BA
Journal of the American Chemical Society 20120307 11
We have developed a multifaceted, highly specific reporter for multimodal in vivo imaging and applied it for detection of brain tumors. A metabolically biotinylated, membrane-bound form of Gaussia luciferase was synthesized, termed mbGluc-biotin. We engineered glioma cells to express this reporter and showed that brain tumor formation can be temporally imaged by bioluminescence following systemic administration of coelenterazine. Brain tumors expressing this reporter had high sensitivity for det ...[more]