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Localization and structural analysis of the ribosomal RNA operons of Rhodobacter sphaeroides.


ABSTRACT: We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. These findings are discussed in relation to the origins of diploidy.

SUBMITTER: Dryden SC 

PROVIDER: S-EPMC332862 | biostudies-other | 1990 Dec

REPOSITORIES: biostudies-other

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Localization and structural analysis of the ribosomal RNA operons of Rhodobacter sphaeroides.

Dryden S C SC   Kaplan S S  

Nucleic acids research 19901201 24


We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and  ...[more]

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