MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration.
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ABSTRACT:
SUBMITTER: Lenco J
PROVIDER: S-EPMC3331852 | biostudies-other | 2012 Feb
REPOSITORIES: biostudies-other
Publications
MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration.
Lenco Juraj J Lan Renny R Edwards Nathan N Goldman Radoslav R
Proteome science 20120204 1
Naturally occurring native peptides provide important information about physiological states of an organism and its changes in disease conditions but protocols and methods for assessing their abundance are not well-developed. In this paper, we describe a simple procedure for the quantification of non-tryptic peptides in body fluids. The workflow includes an enrichment step followed by two-dimensional fractionation of native peptides and MS/MS data management facilitating the design and validatio ...[more]
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